The CO adduct of yeast cytochrome c oxidase. Mössbauer and photolysis studies.
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چکیده
منابع مشابه
Cytochrome c Oxidase from Bakers’ Yeast
Cytochrome aa3 was purified 35to 40-fold from submitochondrial particles of commercial bakers’ yeast. The purification procedure involved solubilization of the enzyme with cholate, fractionation with ammonium sulfate, and chromatography on DEAE-cellulose in the presence of Triton x-100. The purified, active enzyme contained approximately 10 nmoles of heme a per mg of protein and was free of oth...
متن کاملCytochrome c Oxidase from Bakers’ Yeast
Purified cytochrome c oxidase from bakers’ yeast can be resolved into six polypeptide bands by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The apparent molecular weights of these components are I, 42,000; II, 34,500; III, 23,000; IV, 14,000; V, 12,500; and VI, 9,500. (Although Component V actually consists of two distinct polypeptide species, it will be regarde...
متن کاملChapter 10 Purification of the cytochrome C reductase/cytochrome C oxidase super complex of yeast mitochondria.
The protein complexes of the respiratory chain interact by forming large protein particles called respiratory supercomplexes or "respirasomes". Biochemical characterization of these particles proved to be difficult because of their instability. Here we describe a strategy to isolate and characterize the cytochrome c reductase/cytochrome c oxidase supercomplex of yeast, also termed the III + IV ...
متن کاملElectron paramagnetic resonance studies on the cytochrome oxidase from yeast.
I M. D. KAMEN, R. G. BARTSCH, T. HORIO AND H. DE KLERK, in S. P. COLO\VICK AND N. O. tXAPLAN, Methods in Enzymology, Vol. VI, Academic Press, New York, I963, p. 391. 2 T. HORIO AND M. D. KAMEN, Biochim. Biophys. Acta, 48 (I96I) 266. 3 G. COHEN-BAZIRE, ~V. R. SISTROM AND R. Y. STANIER, J . Cellular Physiol., 49 (1957) 25. 4 R. G. BARTSCH, in H. (;EST AND A. SAN PIETRO, Bacterial Photosynthesis, ...
متن کاملStudies on Cytochrome Oxidase. Vi. Kinetics of the Aerobic Oxidation of Ferrocytochrome C by Cytochrome Oxidase.
The rate of reaction can be determined by measuring the rate of either oxygen consumption or ferrocytochrome c oxidation. When the rate of oxygen consumption is measured either manometrically or polarographically, a mixture of a limited amount of cytochrome c and an excess of reducing agent, instead of excess ferrocytochrome c, is used as an electron donor system. In this case the true electron...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)37581-7